Two Protocols

Act I: Cell Encapsulation

 

 

Act II: Growth in selective medium

 

 

Act III - IV: Long incubation and bead fusion

35 min - discussion

 Act I : cell encapsulation

"Lethal encounter"
"Shine a light"

GMO

Wild-type

Subgroup 1

Subgroup 2

Subgroup 1

Subgroup 2

GMO

 Act II : Growth in liquid medium

GMO

GMO

A

Wild-type

B

B

C

Growth medium

Growth medium

+ Antibiotic 

+Arabinose

Growth medium

+ Antibiotic 

+Arabinose

Growth medium

+ Antibiotic 

 

 Act III : Encounter in hydrophobic phase

SHAKE THAT TUBE!

Incubate plate overnight